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Products & Services / Cell Culture

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Cell Culture

GVK BIO has well-established mammalian Cell Culture labs, with state-of-the-art infrastructure supporting mini to large scale culture preparations using cell factories. Our vast experience in expressing the drug targets in mammalian systems enables us to express and validate more than 30 GPCRs, various ion channels and transporters.

  • We have the capability of performing transient and stable cell line generation
  • We have extensive experience in various human and animal cell lines for stable and transient expression of target proteins
  • Our expertise includes protein expression in HEK293, AD293, CHO, MDCK cell lines
  • We have stably expressed more than 40 functionally active target proteins
  • Our validated stable cell lines include GPCR, ion channels and transporters
  • Expressed proteins are routinely assayed for detection of expression levels and also used for screening potential drug candidates

Baculovirus-insect Cell Expression Systems

Baculovirus-insect Cell Systems are considered a good system for recombinant glycoprotein production due to the eukaryotic nature of the host. We have established a standard optimization procedure to determine the conditions for expressing soluble proteins using a Bac-to-Bac expression system. The variables that we may alter to increase the efficiency and productivity of baculovirus expression are:

  • Plaque purification of recombinant viruses
  • Comparison of expression level of different infection parameters: MOI=1, 2, 5 and 10
  • Comparison of expression level of different harvest times: 72, 96, and 120 hours after infection
  • Comparison of expression level of different infection cell densities

To summarize, we can provide:

  • Functionally active human protein up to 500 mg from E.coli
  • Up to 100 mg of human protein from insect cell culture
  • Stable cell lines expressing protein of interest – 5 billion cells/week
  • Use of cell stackers and cell factories for large number of cell production
  • Use of wave bioreactor for insect Cell Culture
  • Large scale membrane preparation for Radio ligand binding assays