Bioanalyses

Technology

  • Q-Tof
  • MS/MSn
  • UV/PDA
  • Fluorescence
  • 96/384/Tube format

Software

  • Discovery Quant 2.2
  • Analyst 1.7
  • Light Sight 2.2.1/ACD
  • Phoenix 7.0
  • Graph Pad Prism 6.0
  • Watson LIMS 7.3

Analytical Method

  • Cartridge/MS Column Switching
  • Protein Precipitation/Liquid Extraction/Solid Phase Extraction
  • Cassette/Discrete Analysis
  • Rapid Fire-High Throughput analysis
  • Matrices: Blood/Plasma/Serum/ tissues/urine/bile/feces/cell culture samples etc

Types of Compounds (small and large molecules)

Endogenous Compounds

  • Amino acids
  • Bile acids
  • Catecholamines
  • Steroids
  • Eicosanoids
  • Lipids
  • Racematic

Natural Compounds

  • Pentacyclic triterpenoids & Polyphenols
  • Boswellic acid
  • Betulinic acid
  • Ursolic acid
  • Fisetin
  • Quercetin
  • Rutin
  • Withaferin (Steroidal lactone)
  • Glucosamine (Amino sugar)

Also, Protac, Nucleotides, Nucleosides, Peptides, Proteins, Biomarkers

Protein fingerprinting protein work flow (Integral mass identification, peptide mapping, glycan analysis, estimations; separation and estimation of deuterated Enantiomers. For quantitative bioanalyses about 100 microl of matrix is required with a turnaround time of 3 days.

Any study including mass spectrometry (MS) assays (such as stability, permeability, protein binding) requires both molecular weight (MW) and formula weight (FW) of each tested compound, even if they are the same. The FW is required to prepare the stock solution of each compound at the correct concentration. The MW gives indication on which expected molecular ion to look for in our LC-MS screen assay.

  • Chemical structure is required for Metabolite characterization
  • Chemical structure or list of the ionizable groups is required for pKa
  • Assuming a molecular weight < 500 g/mol and assays tested at default concentrations (including retest)

Using advanced LC-MS-based techniques for metabolite profiling and structural identification, the Met ID team works in tandem with the entire DMPK unit to provide the information you need to take your compound from concept to candidate.

Brochure

ADME – DMPK

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Whitepaper

A Novel Cell-based Screening Assay for Cholesterol Biosynthetic Pathway Inhibition Using the RapidFire HTMS Platform

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Whitepaper

Pharmacokinetic Strategies in CNS Drug Discovery

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