Non-Alcoholic Steato Hepatitis (NASH) is a silent disease with very few symptoms and can be only detected by a liver biopsy. NASH causes liver inflammation which in turn damages the liver cells leading to dysfunction of the liver.
One of the most commonly used preclinical NASH mode is developed by inducing Amylin, which is 37-residue peptide hormone
Below are few diet models for Preclinical AMLN NASH
Cholesterol and Chocolate Diet – A diet rich in food with high cholesterol combined with chocolate helps the formation of steatosis, inflammation, and fibrosis. Deposition of fat around the liver starts usually from the 6th week. In 24th the hepatocellular ballooning around the liver, which is similar to human NASH can be observed. Histopathological features can be accelerated further by adding cocoa butter to the diet.
High Fat (HF) Diet – A high fat diet when fed to rat, like humans the liver of the rat cannot process the whole input of fat. Rats fed this type of diet showed high level of plasma insulin levels reflecting insulin resistance and developed marked panlobular steatosis & the hepatic lipid concentrations of these rats were twice that of normal levels. By following this diet human NASH like symptoms develop in the 9th week.
Fructose Rich Diet – high fructose diet is an ideal diet for inducing metabolic syndromes in experimental rats. Rats fed with high fructose diet for 5 weeks helps observe significantly higher macrovesicular steatosis and intralobular inflammation.
High Fructose/Glucose in Drinking Water (HF/G) – This type of diet helps in a remarkable increase in total body weight, subcutaneous and visceral adipose tissue, macrosteatosis with a nearly seven times increase in triglyceride and FFA content, accompanied with marked hepatocellular injury, inflammatory responses, fibrosis and insulin resistance at the same time it identical to human NASH in histopathology, metabolic, and adipokine profiles.