Peptide
Chemistry
Solutions

Synthesis and Characterization

With excellent infrastructure facilities, our capabilities lie in synthesizing diverse peptides in a short time with high purities. While our peptide chemistry team specializes in synthesis of linear, cyclic, staple and modified peptides using solution, solid or hybrid technology platforms, our biology solutions team specializes in refolding synthetic peptides into biologically active proteins which are provided to our customers in custom formulation buffers. Our expertise allows us to fold and purify proteins up to gram scales with high purity. In addition, the integrity of the product is characterized by biophysical/biochemical techniques. GVK BIO’s assay development team, which has a track record in developing over 500 assays, has capabilities to validate the folded protein using custom-designed functional assays.

Chemistry Capabilities

  • Linear peptides greater than 100mers
  • Support to supply mg to Kg scale
  • Reactions at ambient and elevated temperature

Biology Capabilities

  • Screening optimal conditions for bond rearrangement and protein folding
  • Screening for optimal folded protein purification parameters
  • Folding and purification from milligram to gram scale
  • Protein modifications such as PEGylation, biotinylation, etc
  • Biophysical/biochemical characterization (peptide mapping/ fingerprinting, mass spectrometry analysis, circular dichroism)
  • Quality control analysis (protein aggregation, endotoxin detection) suited towards your drug discovery needs

Infrastructure

  • Automated peptide synthesizer
  • 48-Mini block and work stations
  • Large jacketed reactors for scale-up of peptides
  • Centrifuge, lyophilizer and prep purification instruments
  • Large scale clarification and concentration using AKTA Flux 6
  • Large scale purifications on our AKTA Avant and AKTA Start platforms
  • Shimadzu Nexera Ultra High-Performance Liquid Chromatograph for analytical experiments

N-terminal and Side-Chain Modifications:

  • PEGylation
  • Acetylation, acylation (e.g. lipopeptides)
  • Biotinylation
  • Phosphorylation (phosphoserine, tyrosine and threonine), sulfation and glycosylation reaction
  • Introduction of maleimido groups, chelating moieties, chromophores and fluorophores, etc

C-Terminal Modifications:

  • Amidation (peptide amide)
  • Peptide alcohols and aldehydes
  • C-terminal esters and thioesters

Cyclizations:

  • Single or multiple disulfide bridges
  • Head-to-tail cyclisation
  • Side chain cyclisation (e.g. lactam bridge, thioether)
  • Hydrocarbon-stapled peptides

Conjugates:

  • Conjugation with linker, biomolecules, carbohydrates, heterocycles, steroids, etc., with spacer, amino acids and others
  • Modification of peptide bond (site specific phosphorylation from azide) in the final molecule

Brochure

Discovery Chemistry Solutions

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Brochure

Extractables and Leachables Solutions

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Whitepaper

Flow Chemistry vs. Batch Processes

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