Standalone solution integrated with assay development and screening
A valuable partner to depend on from gene to protein studies, GVK BIO provides customized reagent generation solutions as recombinant proteins expressed in various expression hosts.
Established track record of delivering over 60 active recombinant proteins of highest purity with customized support towards characterization by various biochemical and biophysical techniques
Experience in generating approximately 50 cell lines
Seamless integration of cell culture, purification, product analysis, and characterization ensuring delivery of a complete package of purified products along with associated biochemical and analytical data
Extensive expertise across all purification strategies throughout the program in achieving highly purified active proteins employing the ÄKTA explorer platform
Challenging genetic manipulation to protein expression in an aggressive timeframe
High throughput recombinant protein expression and purification platforms for optimized target yield and purity
Characterization and validation of proteins by analytical SEC, IEX, RP, Western, ELISA, BIAcore, FACS, IEF, 2D and glycan analysis by HPLC
Culturing capacities up to 20 liters using shake flasks, wave bioreactors and bioreactors
A well-established, dedicated BSL2 lab facility for handling lentiviral and adenoviral based expression systems
Reagent generation solutions:
Molecular Biology Solutions
Customized expression construct generation using an array of gene synthesis and manipulation tools
Extensive experience in generation of expression variants by site directed mutagenesis as well as in design and construction of fusion proteins
Construction of cDNA libraries
Plasmid DNA preparations from mini to giga scale in low to high throughput formats
Protein Expression Solutions
In-depth experience in recombinant protein expression in expression hosts such as E. coli, as well as in insect and various mammalian cell lines
Immense expertise towards the expression of a diverse array of recombinant soluble (cytosolic and secretory) proteins, membrane proteins as well as refolding proteins to their active conformation from bacterial inclusion bodies. Proficiency in baculovirus, adenoviral and lentiviral expression systems
Expression of multiprotein complexes using a dual promoter or bi-cistronic approach (2A peptides/IRES) and other co-expression strategies
Handling of culture volumes up to 20 liters
Protein Purification Solutions
We offer extensive expertise across all purification techniques using the ÄKTA Explorer platform.
Extraction and clarification of large culture volumes for intracellular and secretory proteins
Expertise in purification of proteins with all-encompassing purification resin chemistries and strategies
Processing capacity of 50 liters of culture volume
Protein purification strategies focused on purity, yield and recovery
Endotoxin clearance procedures ensuring minimal EU/mL in deliverables
Functionally validated and well characterized proteins in customized buffers
Cell Line Generation
GVK BIO has well-established mammalian cell culture labs with up-to-date infrastructure supporting small to large scale culture preparations using cell factories.
Vast experience in expressing drug targets in mammalian systems, including validation of more than 30 GPCRs, various ion channels and transporters
Capability of performing transient and stable cell line generation
Expertise in protein expression in HEK293, AD293, CHO and custom cell lines
Stably expressed more than 40 functionally active target proteins
Functional characterization of generated cell lines using various assay platforms