the top 4 reasons to chose our DMPK Services
Our DMPK team is geared up for quick response time.
2Quality and Depth
Data from our DMPK studies is validated and used by clients from the pharmaceutical, biotechnology, agrochemical, tobacco, cosmetics, health care companies and academic organisations. A number of different DMPK reporting options are available.
3Quick Turnaround Time
For most of the core DMPK services is within 3-5 working days. In fact, the turn around time for our DMPK studies is one of the quickest in the industry.
Our highly trained Principal DMPK Scientists on hand to explain results and suggest the most appropriate experimental strategy. Extended support is provided by the DMPK team to the customers in their drug discovery and development process.
For drugs to be effective they must be able to reach their targets in efficacious concentrations.
A number of physicochemical parameters obtained in the following assays are used to assess the potential utility of compounds as therapeutic agents:
- Aqueous solubility (pION, Kinetic and Thermodynamic)
- Partition coefficient (log D) BBB and GIT
- Ionisation constant (pKa) with spectrophotometer
- Chemical stability
The preferred route of drug delivery is oral administration. Intestinal membrane permeability is a critical characteristic that determines the extent and rate of drug absorption and ultimately affects the bioavailability. The human colon adenocarcinoma cell line, Caco-2, and Madin-Darby canine kidney cell line, MDCKII, are used as an alternative in vitro permeability model. PAMPA (parallel artificial membrane permeability assay) is also used to determine the passive permeability of the compounds.
- PAMPA: GIT/BBB/skin
- Caco-2 (Pgp efflux)/MDCK permeability/BCRP
- Skin and buccal permeability by Franz Diffusion Method
- Ongoing Developments
- MDCK -Transfected ABC transporters (stable cell lines)
- SLC transporters: OATP1B1, OATP1B3 and OCT1 (Hepatic)
- OAT1, OAT3 & OCT2 (Renal)
GVK BIO is a specialist provider of DMPK studies and provide a range of in vitro protein binding studies.The free drug concentration is more closely related to the activity of the drug than the total plasma concentration. Drugs with low protein binding have a large volume of distribution and they can equilibrate rapidly with tissues. High protein binding causes a small volume of distribution, slow equilibration with tissues, and are usually a predictor of elimination by metabolism. We employ Rapid Equilibrium Dialysis approach to measure plasma as well as other matrices protein binding taking Warfarin as the reference control.
Knowledge of the partitioning behaviour of a therapeutic compound into red blood cells is important to the interpretation and understanding of a compound’s pharmacokinetic profile. A high partitioning ratio may also lead to the accumulation of the compound in red blood cells and therefore, this parameter serves as an indicator of potential hematotoxicity. We use fresh blood to conduct this study taking chloroquine as the reference control.
Other Specialised Assays